Low Melting Point Agarose 100G - Molecular Biology Grade, P05-SR02-100
INR 45995
Price Details
Price
M.R.P. INR 45995
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Highly purified agarose with very low EEO values certified by strict quality control test procedures.
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Product Details
| Item Weight | 0.22 lbs (100 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for gel electrophoresis, especially for analyzing small DNA fragments and nucleic acids.
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Researchers using PCR
Effective for applications that require low melting point agarose in gel preparation for PCR product analysis.
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Food Industry
Can be used in food testing for gel formation and texture analysis due to its low melting point properties.
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High Temperature Processes
Not suitable for applications requiring high temperature stability as it melts at lower temperatures.
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Non-Biological Uses
May not be appropriate for uses outside molecular biology, such as commercial product formulations.
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Large Gel Applications
Less effective for large gel runs where high gel strength is necessary; low melting point could impact integrity.
Product Description
Low Melting Point Agarose 100G - Molecular Biology Grade, P05-SR02-100
Customer Questions & Answers
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Question:
What is Low Melting Point Agarose used for?
Answer: Low Melting Point Agarose is primarily utilized in molecular biology for applications where low gel temperatures are crucial, such as separating DNA fragments or in cloning techniques. Its low melting point allows researchers to easily manipulate samples without compromising the integrity of nucleic acids. This agarose is ideal for preparing gel electrophoresis for sensitive assays or purification methods, especially when heat-sensitive materials are involved. -
Question:
Can I use Low Melting Point Agarose for protein analysis?
Answer: While Low Melting Point Agarose is predominantly designed for DNA and RNA applications, it can also be used for protein analysis, particularly in situations where gentle handling is required. Its unique properties allow for the recovery of proteins post-separation without denaturation. This makes it suited for applications like mass spectrometry where maintaining protein structure is vital. -
Question:
What concentration of Low Melting Point Agarose should I use?
Answer: The concentration of Low Melting Point Agarose generally ranges from 0.5% to 2%, depending on the size of the DNA fragments being analyzed. A lower concentration is advantageous for larger fragments, as it allows for better resolution, while a higher concentration is beneficial for smaller fragments. For optimal results, it’s essential to calibrate your concentrations based on the specific requirements of your experiment. -
Question:
How should I store Low Melting Point Agarose?
Answer: Low Melting Point Agarose should be stored in a cool, dry place, ideally at room temperature, and away from direct sunlight. It’s best to keep it in a sealed container to avoid moisture absorption. Proper storage ensures the agarose retains its integrity and functionality for future experiments, providing reliable results. -
Question:
Is Low Melting Point Agarose suitable for high-resolution applications?
Answer: Yes, Low Melting Point Agarose is particularly suitable for high-resolution applications due to its fine gel structure that allows for excellent separation of DNA and RNA fragments. This makes it indispensable for applications like sequencing and genotyping where precision is key. Researchers prefer it for situations requiring sensitive detection and purification of nucleic acids. -
Question:
What is the melting temperature of Low Melting Point Agarose?
Answer: The melting temperature of Low Melting Point Agarose usually hovers around 65-70°C. This low melting point is beneficial as it enables the agarose to be liquefied at lower temperatures for gel preparation, making it easier to handle and pour without damaging sensitive samples. This characteristic is essential for applications involving fragile biological molecules. -
Question:
Can I reuse Low Melting Point Agarose gels?
Answer: Reusing Low Melting Point Agarose gels is typically not recommended. Once a gel has been used for electrophoresis, its structure can be compromised, leading to poor resolution and unreliable results. It’s best to prepare fresh gels for each experiment to ensure optimal performance and accurate analysis of your samples. -
Question:
What are the differences between Low Melting Point Agarose and regular agarose?
Answer: The primary difference lies in their melting points: Low Melting Point Agarose melts at lower temperatures compared to standard agarose. This characteristic allows for easier manipulation of the gel post-electrophoresis. Regular agarose is more suited for routine applications where the gel is not intended to be melted after use. The choice between the two depends on the specific requirements of your molecular biology techniques. -
Question:
Is Low Melting Point Agarose safe to use?
Answer: Low Melting Point Agarose is considered safe to use when handled following standard laboratory safety protocols. It is non-toxic and poses minimal risk when used appropriately. Always wear gloves and safety goggles while handling agarose and follow your lab's safety procedures to ensure a safe working environment. -
Question:
Where can I buy Low Melting Point Agarose 100G in India?
Answer: You can purchase Low Melting Point Agarose 100G - Molecular Biology Grade, P05-SR02-100 from Ubuy. They offer a variety of laboratory supplies, including this specialized agarose, ensuring you receive a high-quality product for your molecular biology applications.
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INR 45995
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Features & Benefits
- Optimal concentration between 0.7-2% for versatile applications.
- GQ certified for guaranteed genetic quality.
- Low DNA/RNA binding for enhanced recovery.
- Excellent gel transparency for improved analysis.
- DNase and RNase free, ensuring sample integrity.
- Ideal for a variety of molecular biology techniques.