What is 1M Tris Buffer, pH 7.5 used for?

1M Tris Buffer at pH 7.5 serves as a crucial component in biochemical and molecular biology applications. It is commonly employed in DNA and RNA extraction, protein purification, and gel electrophoresis. The buffering capability facilitates maintaining a stable pH environment during enzymatic reactions, which is essential for accurate results and effective research. Additionally, its versatility makes it suitable for a variety of laboratory protocols.

How is the 1M Tris Buffer prepared?

To prepare 1M Tris Buffer at pH 7.5, Tris base is dissolved in distilled water and the pH is adjusted using hydrochloric acid (HCl). It's important to filter the solution, typically using a 0.22um filter, to remove any particulates, ensuring sterility and clarity. This process guarantees that your buffer is suitable for sensitive applications requiring sterile conditions, such as cell culture or molecular experiments.

What does 'sterile' mean in the context of 1M Tris Buffer?

The term 'sterile' indicates that the 1M Tris Buffer has been treated to eliminate all viable microorganisms. This ensures that the buffer can be used in critical applications without the risk of contamination affecting experimental results. Sterilization often involves filtration through a 0.22um membrane, which is standard practice in laboratories to maintain a contaminant-free environment, particularly in cell and molecular biology experiments.

Can 1M Tris Buffer be used for protein storage?

Yes, 1M Tris Buffer at pH 7.5 is suitable for protein storage. Its ability to maintain a stable pH is essential in preserving protein structure and function over time. When proteins are stored in this buffer, it helps to prevent denaturation, ensuring they remain active for future experiments. This characteristic makes it a common choice among researchers working with sensitive proteins.

What is the significance of the pH being set at 7.5?

A pH of 7.5 is particularly significant as many biological reactions occur around this pH level. It is optimal for enzyme activity in numerous biochemical assays. This pH range ensures that enzymatic activity is maximized, leading to accurate and reliable results in experiments. Many researchers favor this pH for buffer preparation to align with typical cellular environments.

Is 1M Tris Buffer suitable for electrophoresis?

Absolutely! 1M Tris Buffer at pH 7.5 is widely used in gel electrophoresis applications. When combined with other components, it helps maintain the required pH during the separation of nucleic acids or proteins. This is critical as fluctuations in pH can adversely affect the migration patterns or integrity of the samples, thereby impacting experimental outcomes.

How should 1M Tris Buffer be stored?

1M Tris Buffer should be stored at room temperature, away from direct light to prevent degradation. If you anticipate long-term storage, refrigeration is ideal. Proper sealing in a sterile container can prevent contamination and ensure that the buffer remains viable for future use. Regular checks for clarity and any changes in color are advisable to ensure the buffer's integrity.

What applications require sterile 1M Tris Buffer?

Sterile 1M Tris Buffer is crucial for applications involving cell culture, transfection, and any experimental setups where contamination could impact results. It is particularly beneficial in molecular biology techniques, such as PCR, where maintaining sterility is vital. Researchers often choose sterile buffers to ensure consistent outcomes and reliability in sensitive experimental conditions.

Can I use 1M Tris Buffer for other pH levels?

While this specific buffer is formulated at pH 7.5, adjusting Tris buffer to other pH levels is possible by altering the concentration of HCl or NaOH added during preparation. This creates buffers with varying pH levels suitable for different applications, which is beneficial in a laboratory setting. It allows researchers the flexibility to create a tailored buffering solution that best fits their experimental needs.

Where can I buy 1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - 500mL?

You can purchase 1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - 500mL from Ubuy. They offer a reliable platform for sourcing various laboratory reagents and supplies, ensuring you find the products you need with convenience and ease in India.

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Is 1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - Available to Shop Online in India?

Yes, at Ubuy India this product is available for you to shop at a reasonable price. The 1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

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UFC Bio

1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - 500mL

UFC Bio

Item #: 145396059

1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - 500mL

99% of respondents would recommend this to a friend

Item #: 145396059

INR 4848

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What Stands Out

High Purity

This 1M Tris buffer is sterile and filtered at 0.22 microns, ensuring contamination-free experiments and reliable results for sensitive applications in laboratories.

Optimal pH Stability

Designed with a pH of 7.5, this buffer maintains optimal conditions for biological and chemical reactions, making it ideal for a variety of research applications.

Convenient Size

The 500mL packaging offers ample volume for multiple experiments, reducing the need for frequent reordering and ensuring labs stay stocked with essential reagents.

Product Details

Container Type	Bottle
Item Form	Liquid
Volume	500 ml
Brand Name	UFC Bio
Manufacturer	UFC Bio
UPC	791689523668
Included Components	1X Tris Buffer, pH 7.5
Part Number	BTHCL75-1M-500ML
Brand	UFC Bio
Item Volume	500 ml
Package Information	Bottle
Item Weight	1.1 lbs (500 grams)

What's in the box

1X Tris Buffer, pH 7.5

Who Should Buy?

Biotechnology Labs
Essential for experiments requiring a stable pH environment in DNA and protein analysis and other applications.
Molecular Biology
Ideal for buffering solutions in various molecular biology techniques, including electrophoresis and PCR preparation.
Clinical Research
Useful in sterile conditions for clinical applications, ensuring reliable results in diagnostic and therapeutic studies.

Non-sterile Environments
Not suitable for situations where sterility is not maintained, leading to contamination risks in sensitive experiments.

Product Description

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Question: How to Shop 1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - Online From Ubuy?

Answer: It’s easy to shop 1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is 1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - Available to Shop Online in India?

Answer: Yes, at Ubuy India this product is available for you to shop at a reasonable price. The 1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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1M Tris Buffer, pH 7.5-0.22um Filtered - Sterile - 500mL

INR 4848

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INR 4848

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